禽呼肠孤病毒蛋白质启动PI3K/Akt信号通路的分析

doi:10.11843/j.issn.0366-6964.2015.09.017

Abstract

Abstract:

This study was conducted to find out the avian reovirus (ARV) proteins which can activate the phosphatidylinositol 3-Kinase-dependent Akt(PI3K/Akt) pathway.According to the analysis of amino acid sequence of ARV proteins，the σA，σNS，μA，μB and μNS were selected as the putative proteins which mediated the activation of PI3K/Akt pathway.Reconbinant plasmids，σA-pcAGEN，σNS-pcAGEN，μA-pcAGEN，μB-pcAGEN and μNS-pcAGEN were constructed and transfected into Vero cells，and the expression of the target genes were identified by immunofluorescence test and Western blot.The phosphorylated Akt (P-Akt) profile of transfected cells were examined by flow cytometry and Western blot.The results showed that σA，σNS，μA，μB and μNS genes were expressed in the Vero cells，and the expression of P-Akt of σA-pcAGEN and σNS-pcAGEN groups increased markedly.The PI3K inhibitor LY294002 could inhibit the expression of the P-Akt of σA-pcAGEN and σNS-pcAGEN groups.The results indicate that σA and σNS protein could activate the PI3K/Akt pathway.

CLC Number:

S852.659.4

XIE Li-ji，XIE Zhi-xun，HUANG Li，XIE Zhi-qin，DENG Xian-wen，LIU Jia-bo，LUO Si-si，HUANG Jiao-ling，ZENG Ting-ting，ZHANG Yan-fang，WANG Sheng. The Study of the Activation of PI3K/Akt Pathway by the Protein of Avian Reovirus[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(9): 1613-1619.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/10.11843/j.issn.0366-6964.2015.09.017

https://www.xmsyxb.com/EN/Y2015/V46/I9/1613

References

［1］VIVANCO I，SAWYERS C L.The phosphatidylinositol 3-Kinase AKT pathway in human cancer［J］.Nat Rev Cancer，2002，2(7)：489-501.
［2］KAY B K，WILLIAMSON M P，SUDOL M.The importance of being proline：the interaction of proline-rich motifs in signaling proteins with their cognate domains［J］. FASEB J，2000，14(2)：231-241.
［3］PAWSON T.Protein modules and signalling networks［J］.Nature，1995，373(6515)：573-580.
［4］STREET A，MACDONALD A，CROWDER K，et al.The Hepatitis C virus NS5A protein activates a phosphoinositide 3-kinase-dependent survival signaling cascade［J］.J Biol Chem，2004，279(13)：12232-12241.
［5］SONGYANG Z，SHOELSON S E，CHAUDHURI M，et al.SH2 domains recognize specific phosphopeptide sequences［J］. Cell，1993，72(5)：767-778.
［6］SHIN Y，LIU Q，TIKOO S K，et al.Influenza A virus NS1 protein activates the phosphatidylinositol 3-kinase(PI3K)/Akt pathway by direct interaction with the p85 subunit of PI3K［J］.J Gen Virol，2007，88(Pt 1)：13-18.
［7］ZHANG B，SPANDAU D F，ROMAN A.E5 protein of human papillomavirus type 16 protects human foreskin keratinocytes from UV B-irradiation-induced apoptosis［J］.J Virol，2002，76(1)：220-231.
［8］LABRADA L，BODELÓN G，VIÑUELA J，et al.Avian reoviruses cause apoptosis in cultured cells：viral uncoating，but not viral gene expression，is required for apoptosis induction［J］.J Virol，2002，76(16)：7932-7941.
［9］SHIH W L，HSU H W，LIAO M H，et al.Avian reovirus sigmaC protein induces apoptosis in cultured cells［J］.Virology，2004，321(1)：65-74.
［10］LIN P Y，LIU H J，LIAO M H，et al.Activation of PI 3-kinase/Akt/NF-κB and Stat3 signaling by avian reovirus S1133 in the early stages of infection results in an inflammatory response and delayed apoptosis［J］.Virology，2010，400(1)：104-114.
［11］LIU H J，LEE L H，HSU H W，et al.Molecular evolution of avian reovirus ：evidence for genetic diversity and reassortment of the S-class genome segments and multiple cocirculating lineages［J］.Virology，2003，314(1)：336-349.
［12］TENG L，XIE Z，XIE L，et al.Sequencing and phylogenetic analysis of an avian reovirus genome［J］.Virus Genes，2014，48(2)：381-386.
［13］XIE Z，QIN C，XIE L，et al.Recombinant protein-based ELISA for detection and differentiation of antibodies against avian reovirus in vaccinated and non-vaccinated chickens［J］. J Virol Methods， 2010，165(1)：108-111.
［14］WANG X，ZHANG H，ABEL A M，et al.Role of phosphatidylinositol 3-kinase(PI3K) and Akt1 kinase in porcine reproductive and respiratory syndrome virus(PRRSV) replication［J］. Arch Virol，2014，159(8)：2091-2096.
［15］BARBER G N.Host defense，viurses and apoptosis［J］.Cell Death Differ，2001，8(2)：113-126.
［16］BENEDICT C A，NORRIS P S，WARE C F.To kill or be killed：viral evasion of apoptosis［J］.Nat Immunol，2002，3(11)：1013-1018.
［17］HAY S，KANNOURAKIS G.A time to kill：viral manipulation of the cell death program［J］.J Gen Virol，2002，83(Pt 7)：1547-1564.
［18］KRAJCSI P，WOLD W S.Viral proteins that regulate cellular signalling［J］.J Gen Virol，1998，79(Pt 6)：1323-1335.
［19］D’ANGLEMONT DE TASSIGNY A，BERDEAUX A，SOUKTANI R，et al.The volume-sensitive chloride channel inhibitors prevent both contractile dysfunction and apoptosis induced by doxorubicin through PI3kinase，Akt and Erk 1/2［J］.Eur J Heart Fail，2008，10(1)：39-46.

[1]	CHEN Shuyu, ZHU Xuejiao, ZHOU Jinzhu, TAO Ran, ZHANG Xuehan, Suolangsizhu, Gongga, LI Bin. Inhibition of Porcine Rotavirus in vitro Replication by Guanylate-binding Proteins GBP1 and GBP2 [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 245-257.
[2]	LI Huihui, DONG Keer, LIU Xinbo, ZHANG Chunxiao, MA Chao, CHEN Liping, ZHONG Qi, YAO Gang, MA Xuelian. Establishment and Application of Rapid Detection Method for Bovine Norovirus and Bovine Rotavirus Dual RAA-LFD [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 406-412.
[3]	LI Xiaohan, YANG Fuchun, LIU Rui, GAO Li, CUI Hongyu, ZHANG Yanping, LIU Changjun, QI Xiaole, WANG Xiaomei, GAO Yulong, LI Kai. Construction of Recombinant Herpesvirus of Turkey Expressing Infectious Bursal Disease Virus VP2 Gene and Analysis of Its Biological Characteristics [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(2): 656-662.
[4]	LI Zhanhong, ZHU Pei, SONG Ziang, LI Zhuoran, YANG Zhenxing, LI Huachun, YANG Heng, LIAO Defang. Establishment and Application of a Group Specific Reverse Transcriptase Loop-mediated Isothermal Amplification Method of Bluetongue Virus [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(8): 2244-2253.
[5]	LÜ Ting, CHEN Hongyin, TANG Cheng, YUE Hua. Detection, Isolation and Identification of Mammalian Orthoreovirus Serotype 2 in Yaks [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(8): 2361-2368.
[6]	LI Zhanhong, SONG Ziang, LIAO Defang, YANG Zhenxing, XIE Jiarui, GAO Xiang, HU Zhongyan, LI Zhuoran, LI Huachun, YANG Heng. Whole Genome Sequence Analyses of Bluetongue Virus Serotype 7 Strain Isolated from Yunnan Province [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(5): 1337-1348.
[7]	LI Maolin, ZHANG Qijin, XU Qingyuan. Global Distribution of Bluetongue [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(4): 881-890.
[8]	YI Huashan, ZHAO Yao, MA Xianping, LI Huan, XIA Yu, ZHU Wenqing, LIU Qianru, CHEN Siqian, CAO Huizhen, YAO Ting, GAO Lixu, ZHANG Jinyang, YANG Fahui, WEI Xiaorong, LI Qianyong, XIE Yuanbing. Establishment and Preliminary Application of Indirect ELISA Based on Recombinant NS4 Protein for Detection of Bluetongue Virus Antibodies [J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(12): 3133-3140.
[9]	YU Kexiang, LIU Cunxia, GONG Xiao, LU Xiao, HU Feng, GUO Xiaozhen, MA Xiuli, LI Yufeng, HUANG Bing, SONG Minxun. Isolation and Identification of a Variant Strain of Reovirus from Broilers [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(5): 1039-1047.
[10]	LI Ran, TANG Cheng, LUO Xue, YUE Hua. Isolation and Gene Sequence Analysis of G6P[11] Bovine Rotavirus HY-1 Strain from Yak [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(3): 592-601.
[11]	YANG Zhenxing, MENG Jinxin, XIAO Lei, ZHU Jianbo, LIAO Defang, GAO Lin, LI Zhanhong, YANG Heng, LI Huachun. Isolation of Epizootic Haemorrhagic Disease Virus Serotype 7 Strain in China for the First Time [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(3): 602-610.
[12]	LI Zhanhong, WANG Jinping, YANG Heng, LIAO Defang, SONG Jianling, GAO Lin, HE Yuwen, LI Huachun. Isolation of Bluetongue Virus Serotype 9 Strain in China for the First Time [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(2): 354-363.
[13]	YANG Heng, XIAO Lei, LI Zhan-hong, MENG Jin-xin, YANG Zhen-xing, Lü Min-na, LIN Xu-hui, LIAO De-fang, NIU Bao-sheng, LI Hua-chun. Sequence Analysis of Palyam Serogroup Virus Isolated in South China from 2012 to 2016 [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(4): 761-770.
[14]	CHI Xiao-juan, SHAO Wen-han, CHEN Ji-long, CHEN Yue-xiang, WANG Song. The Research Advance of Host Immune Response Induced by Avian-origin Reovirus [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(12): 2249-2257.
[15]	HUANG Li, XIE Zhi-xun, XIE Li-ji, HUANG Jiao-ling, XIE Zhi-qin, FAN Qing, DENG Xian-wen, LUO Si-si, ZHANG Yan-fang, ZENG Ting-ting, WANG Sheng, ZHANG Min-xiu. Transcriptomic Expression Profiles of LMH Cells Infected with Avian Reovirus Virulent Strain and Attenuated Vaccine Strain [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(11): 2135-2147.

The Study of the Activation of PI3K/Akt Pathway by the Protein of Avian Reovirus

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments